Modifying Enzymes
New England Biolabs, Inc. EnGen Spy Cas9 HF1 - 500 pmol
EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5'-NGG-3' protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM. EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein.
Enzo Life Sciences p300 (catalytic domain) (human), (recombinant) (100 µg)
Synonyms: E1A binding protein p300, Histone acetyltransferase p300. MW: 45.1 kDa. UniProt ID: Q09472. Source: Produced in E. coli. Catalytic domain (aa 1284-1673) of human p300. Purification: Partially purified by single-step affinity chromatography and gel filtration. Formulation: Liquid. In 50mM TRIS/HCl, pH 8.0, containing 0.1mM EDTA, 10% glycerol.
Enzo Life Sciences Granzyme B (human), (recombinant) (5000 U)
MW: 25.4 kDa. Concentration: 100 U/µl. Formulation: Liquid. In 50mM TRIS, pH 7.4, 100mM NaCl, 10% glycerol. Source: Produced in yeast. Recombinant human granzyme B (aa 21-247). UniProt: P10144. Long Term Storage: -80°C
LGC GENOMICS LLC (Lucigen) Terminator 5'-Phosphate-Dependent Exonuclease, 40U @ 1 U/LA processive 5 to 3 riboexonuclease that specifically digests RNA with 5-monophosphate ends only.
Terminator™ 5-Phosphate-Dependent Exonuclease is a processive 53 exonuclease that digests RNA that has a 5-monophosphate end. It does not digest RNA that has a 5-triphosphate, 5-cap or 5-hydroxyl group.
New England Biolabs, Inc. Thermostable USER III Enzyme - 50 units
Thermostable USER (Uracil-Specific Excision Reagent) III Enzyme generates a single nucleotide gap at the location of a uracil. Thermostable USER III Enzyme is a mixture of Afu Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease IV. Afu UDG catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease IV breaks the phosphodiester backbone at the 3’ and 5’ sides of the abasic site so that base-free deoxyribose is released leaving a 5’ deoxyribose phosphate (dRP) and a 3’-hydroxyl. Thermostable USER III Enzyme also has 3’-diesterase activity which can remove 3’ phosphate, 3’-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. Thermostable USER III Enzyme is optimally active at 65C.
Worthington Biochemical Corporation HYALURONIDASE
Hyaluronidase, 50 ku/ Pack, A partially purified, dialyzed, lyophilized powder. Store at -20degreeC., Source: Bovine Testes, Minimum Activity: =300 USP/NF units per mg dry weight
New England Biolabs, Inc. Thermostable OGG - 500 units
Thermostable OGG is an archaeal 8-oxoguanine (8oxoG) DNA glycosylase which acts both as aN-glycosylase and an AP-lyase. TheN-glycosylase activity releases the damaged purine (8-oxoguanine) from double stranded DNA, generating an apurinic (AP) site. The AP-lyase activity cleaves 3' to the AP site leaving a 5' phosphate and a 3'-phospho-alpha, beta-unsaturated aldehyde. Unlike some other DNA glycosylases, Thermostable OGG specifically recognizes and cleaves only 8oxoG and no other modified bases.
![Biolegend PerCP/Fire™ 780 anti-human CD4 [SK3]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502530554.png-250.jpg)
Biolegend PerCP/Fire™ 780 anti-human CD4 [SK3]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests
PerCP/Fire™ 780 anti-human CD4 [SK3]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests
Discovery Life Sciences HUMAN FMO1 0.5ML@5MG/ML
Human FMO1 SUPERSOMES;Available size: 0.5 ml. Total protein concentration: 5.0 mg/ml. Catalytic assay: methyl p-tolyl sulfide oxidase activity, representative catalytic activity: 10,000 pmol product/(min x mg protein).
STEMCELL Technologies Hyaluronidase, Size: 300000 U
Hyaluronidase is a polysaccharidase consisting of a single polypeptide chain of 450 amino acid residues and contains four disulfide bonds. Hyaluronidase is glycosylated and contains 5% mannose and 2.17% glucosamine (Borders & Raftery). Hyaluronidase cleaves endo-N-acetlyhexosaminic bonds in hyaluronic acid and chondroitin sulfate A and C to tetrasaccharide residues, as hyaluronic acid and chondroitin sulfate are often found in connective tissues. Hyaluronidase is frequently used with other proteases such as collagenase.
New England Biolabs, Inc. Nuclease P1 – 10000 units
Nuclease P1 (from P. citrinum) is a zinc-dependent single-strand specific nuclease which hydrolyzes 3->5 phosphodiester bonds in RNA and ssDNA with no base specificity. Nuclease P1 also exhibits 3-phosphomonoesterase activity. Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer r1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.
STEMCELL Technologies ArciTect™ T7 Endonuclease I Kit, Size: 25 Reactions
ArciTect™ T7 Endonuclease I is the preferred enzyme for detecting genome editing such as insertions or deletions (INDELs) generated by CRISPR-Cas9. ArciTect™ T7 Endonuclease I Kit is comprised of ArciTect™ T7 Endonuclease I and ArciTect™ T7 Endonuclease I Buffer (10X), which have been tested and validated for use with the ArciTect™ CRISPR-Cas9 genome editing system. ArciTect™ T7 Endonuclease I recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA, and, less efficiently, nicked double-stranded DNA. Since the cleavage efficiency is proportional to the number of INDELs created at a specific DNA target, ArciTect™ T7 Endonuclease I Kit is used to estimate gene-editing efficiency in a rapid and cost-effective manner.